Fig. 6

Loss of α-tanycytes. Co-immunofluorescence for the tanycyte marker Vimentin and for Gfap on (a) central and (b) posterior hypothalamic sections of WT and Gnasxl-deficient mice. White arrows mark the general localisation of α-tanycytes, most of which co-express both markers. Asterisks indicate the regions of Gfap-negative, Vimentin-positive β-tanycytes. While double-positive α-tanycyte extensions and cell bodies are strongly reduced, β-tanycytes appear unaffected in the KO mice. White arrowheads indicate remaining Gfap/Vimentin double-positive α-tanycyte processes in the KO samples. The white rectangle on the DAPI images in (a) indicates the position of the higher magnification Gfap/Vimentin images. Scale bars represent 100 μm