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Fig. 2 | Molecular Brain

Fig. 2

From: Lysosomal iron modulates NMDA receptor-mediated excitation via small GTPase, Dexras1

Fig. 2

Chelation of iron modifies the excitability of pyramidal cells and circuit of the hippocampus. a. Whole cell patch clamp of CA1 pyramidal cells shows that 100 μM PIH application induces an increase in spontaneous EPSC frequency from 1.7 ± 0.28Hz to 2.8 ± 0.35Hz (*p < 0.05). On the right, there is no change in IPSC with PIH. Top shows representative traces from an experiment and bottom are bar graphs of cumulative data. b. There is an increase in the evoked EPSP in PIH. On the top is example recordings of whole cell current clamp and bottom is a graph showing population (black) and average data (red) in the WT animals both before and after 100 μM PIH application. PIH causes an increase in the amplitude of the evoked EPSP in CA1 pyramidal cells (Control: 3.7 ± 0.4 mV, PIH: 5.23 ± 0.4 mV, n = 6, p < 0.05). c. Shows grey scale image of a hippocampal slice (on the left) and snapshots of peak responses to stimulation in control (middle) and 100 μM PIH (right) conditions (average of 30 ms). Underneath are the traces of an average response of pixel fluorescence signals over time from a standardized region of CA1 in both conditions. There is an enhancement of the response following PIH application. Population line graphs of the VSD experiments as well as the average (red) confirm that there is a consistent increase in both amplitude of EPSP and time to decay back to baseline (tau) (n = 10, *p < 0.05)

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