Fig. 4
From: Lysosomal iron modulates NMDA receptor-mediated excitation via small GTPase, Dexras1
Iron dependent regulation of hippocampal activation is dependent on NMDA not AMPA. a. Shows an example of whole cell recordings of WT slices during AP5 and AP5 + 100 μM PIH. Blocking NMDA-R with AP5 abolishes the increase in spontaneous EPSCs. Bar graph shows average of 6 experiments (Control: 2.6 ± 0.9Hz, PIH: 2.5 ± 0.9Hz, p = 0.85). b. AP-5 blocks or if anything reduces the PIH induced increase in amplitude of the evoked VSDi EPSP (Control: 3.4 ± 0.7 mV, PIH: 2.7 ± 0.7 mV, n = 5, p = 0.17). c. An example of a VSDi experiment with AP5 treatment (left) and AP5 with 100 μM PIH added (right). The NMDA-R antagonist AP5 blocks the increase in evoked VSDi EPSPs, as shown by the representative traces underneath the snapshots of peak responses. d. VSDi analysis of evoked EPSP in the presence of the DNQX (AMPA and kainate receptor antagonist) in low Mg2+ ACSF, which are conditions favoring NMDA activity (left) and DNQX with PIH on the right. Blocking AMPA and kainate receptors does not have an effect on the VSDi eEPSP. e. Blocking NMDA receptors causes PIH to no longer be able to increase the VSDi eEPSP. However only blocking AMPA receptors still allows for an increase in VSDi eEPSP size (p < 0.05). WT data is shown for comparison