Fig. 6
From: Lysosomal iron modulates NMDA receptor-mediated excitation via small GTPase, Dexras1
Iron chelation dependent increase in excitability is dependent on Dexras1. a. Whole cell patch clamp in Dexras1 KO animals show that there is no effect of chelation on CA1 excitability. PIH has no effect on the frequency of EPSCs in CA1 neurons in Dexras1 KO animals (Control: 1.4 ± 0.3 Hz, PIH: 1.2 ± 0.1 Hz, n = 8, p = 0.13). PIH application does not increase VSDi eEPSP sized in Dexras KO despite using Low Mg2+ ACSF. b. Whole cell patch in pyramidal cells of Dexras1 KO also show no increase in evoked EPSPs when PIH is applied On the top is example recordings of whole cell current clamp and bottom is a graph showing population (black) and average data (red) in the WT animals both before and after 100 μM PIH application (Control: 10.0 ± 2.6 mV, PIH: 8.8 ± 2.3 mV, n = 8, p = 0.27). c. VSDi analysis of Dexras1 KO animals shows that PIH has no effect on the evoked response. An example of a VSDi experiment with Dex KO slices control (left) and with 100 μM PIH added (right). Directly underneath are average responses averaged over 30 ms post stimulus. d. Knocking out Dexras1 causes PIH to no longer have an effect on VSDi eEPSP size