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Fig. 7 | Molecular Brain

Fig. 7

From: Lysosomal iron modulates NMDA receptor-mediated excitation via small GTPase, Dexras1

Fig. 7

The NO pathway and DMT1 (components of the Dexras1 pathway) are necessary for iron to effect neuronal excitability. a. Blocking the NO pathway blocks the PIH induced increase in VSDi eEPEP. An example of a VSDi experiment with L-NAME treatment (left) and L-NAME with 100 μM PIH added (right). The NO pathway antagonist blocks the increase in evoked VSDi EPSPs, as shown by the representative traces underneath the snapshots of peak responses and population data on the right. (Amp: L-NAME: 1.3x10−3 ± 0.1x10−3 %ΔF/Fo, PIH: 1.2x10−3 ± 8.3x10−5 ΔF/Fo, n = 4, p = 0.15). b. Blocking DMT1 with ebselen also blocks the PIH induced increase in VSDi eEPEP. An example of a VSDi experiment with ebselen treatment (left) and ebselen with 100 μM PIH added (right). The DMT1 antagonist blocks the increase in evoked VSDi EPSPs, as shown by the representative traces underneath the snapshots of peak responses and population data on the right (Amp: ebselen: 4.6x10−3 ± 0.5x10−3 %ΔF/Fo, PIH: 4.6x10−3 ± 0.4x10−3 ΔF/Fo, n = 3, p = 0.8). c. Blocking the NO pathway with L-NAME or blocking DMT1 with ebselen, cause PIH to no longer be able to increase the VSDi eEPSP

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