Fig. 6

Cooperative effects of ethanol and peroxide challenges on apoptosis and lysosomal/mitochondrial distribution. At day 7 after exposure to ethanol for 24hr or 7d, iPS cells were exposed for 14hr to 5 or 10 μM H₂O₂ and immunostained with antibodies against Oct4 and Casp3 (a), or stained with Mitotracker^™ and Lamp1 (b). Both single treatments with ethanol and H₂O₂ alter the normal patterns of the cell, but a remarkable enhancement of the effects observed following a single challenge is evident following a double challenge. Scale bars: 50 μm (a), 10 μm (b)