Fig. 2

M2 macrophages are increased in the ipsilateral brain after ICH. a-e mRNA expressions of M2 markers (Arg-1 and Ym1) or M1 markers (CD16, CD86 and iNOS) in the ICH mice brains were measured using real-time RT-PCR. Total RNA was isolated from ipsilateral hemorrhagic tissue and used for quantitative real-time RT-PCR (n = 3 per group, **p < 0.01, ***p < 0.001). f Representative images of Arg-1 immunofluorescence (red) in the CX3CR1 ^+/GFP mice brain sections obtained from ICH or sham-control mice. Arg-1 immunofluorescence merged with myeloid cell-specific GFP signals is denoted (arrows) (Scale bar, 100 μm). g Dissociated cells from the injured tissue were obtained 1, 3, and 7 days after ICH injury and were used for flow cytometry with anti-CD11b-FITC, −CD45-PE, and -CD206-APC antibodies. Representative flow cytometry histograms show CD206⁺ cells gated on CD11b⁺/CD45⁺ populations. The flow cytometry data are representative of three independent experiments