Fig. 3
Depletion of peripheral monocytes in ICH injury. a Schematic diagram of the myeloid cell depletion protocols using Cl2MDP. b Blood cells obtained from saline (black line)- or Cl2MDP-treated ICH mice (gray line) were stained with anti-CD11b-FITC antibody and analyzed with flow cytometry. c Cell suspensions obtained from saline- or Cl2MDP-treated mouse brain were stained with anti-CD11b-FITC and anti-CD45-PE antibody and analyzed with flow cytometry. Representative flow cytometry dot plots are shown. d ICH injured brain sections were collected and stained with anti-CD68 antibody. Representative images of three independent experiments are shown (Scale bar, 50 μm). e One day after Cl2MDP injection, mice were subjected to ICH, and the brains were sectioned and stained with cresyl violet at 3 dpi. Representative pictures are shown (Scale bar, 1 mm). f Hemorrhagic injured volumes were quantified and presented in a graph (n = 4 per group, *p < 0.05). g, h After Cl2MDP or saline i.p. injection, mice were subjected to ICH, and neurological outcomes were evaluated by focal deficit and sticky tape removal time at 1 and 3 days after ICH (n = 5 for saline and Cl2MDP groups in ART; n = 4 for saline group, n = 3 for Cl2MDP group in focal deficit, *p < 0.05). Data are expressed as mean ± SEM