Fig. 9

Effects of NONRATT021972 siRNA on the ATP-activated current in HEK293 cells transfected with pEGFP-hP2X₇ plasmid. NONRATT021972 siRNA reduced the ATP-induced currents in HEK293 cells expressing the hP2X₇ receptor. a Whole cell patch clamp recordings were performed on the HEK293 cells expressing P2X₇ alone. The plasmid was tagged with EGFP to enable the detection of the transfected cells. Inward currents were evoked by 100 μM ATP at a holding potential of −60 mV. The currents can be inhibited by 10 μM A-438079 (a selective antagonist of the P2X₇ receptor). b ATP activated currents were recorded in the HEK293 cells transfected with P2X₇ alone or cotransfected with NONRATT021972 siRNA. c The histogram shows the normalized, mean peak current (mean ± SD, n = 12, ^* p < 0.05)