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Fig. 3 | Molecular Brain

Fig. 3

From: Histamine 1 receptor-Gβγ-cAMP/PKA-CFTR pathway mediates the histamine-induced resetting of the suprachiasmatic circadian clock

Fig. 3

Gβγ-cAMP/PKA signaling pathway plays a crucial role in H1R agonist-elicited Ca2+ rise and Cl fluxes. a Summary graphs showing the effects of the Gβγ blocker gallein on H1R agonist-elicited increase in [Ca2+]i. The bar charts indicate the mean (± SEM) peak Ca2+ responses. **: p < 0.001 [n = 47 neurons from 3 mice, t(46) = 10.409, paired t-test]. b Summary graphs showing the effects of gallein on H1R agonist-induced Cl efflux [left panel, n = 21 cells from 4 mice, t(20) = 6.783, p < 0.001, paired t-test] and influx [right panel, n = 50 cells from 4 mice, t(49) = −14.644, p < 0.001, paired t-test]. The bar charts indicate the mean (± SEM) peak Cl responses. **: p < 0.001. c Summary graphs showing the effects of the PKA inhibitor H89 on H1R agonist-elicited increase in [Ca2+]i. The bar charts indicate the mean (± SEM) peak Ca2+ responses. **: p < 0.001 [n = 90 neurons from 3 mice, t(89) = 7.272, paired t-test]. d Summary graphs showing the effects of H89 on H1R agonist-induced Cl efflux [left panel, n = 277 cells from 8 mice, t(276) = 19.895, paired t-test] and influx [right panel, n = 90 cells from 8 mice, t(89) = −11.458, paired t-test]. The bar charts indicate the mean (± SEM) peak Cl responses. **: p < 0.001. The symbols connected by lines in (a-d) denote data from the same cells

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