Fig. 4

The CFTR inhibitor CFTR_inh-172 suppresses histamine-elicited Cl⁻ efflux and Ca²⁺ rise in SCN cells. a Traces from Cl⁻-imaging experiments that show the inhibitory effects of CFTR_inh-172 on 2-pyridylethylamine (H1R agonist)-induced Cl⁻ efflux in an SCN cell. b Summary graph showing the effect of CFTR_inh-172 on H1R agonist-induced Cl⁻ efflux (n = 41 cells from 4 mice; left panel) and influx (n = 38 cells from 4 mice; right panel) in SCN cells. c Summary graph showing the effect of CFTR_inh-172 on histamine-elicited increase in [Ca²⁺]_i in SCN neurons (n = 31 neurons from 3 mice). The bar charts in (b) and (c), respectively, indicate the mean (± SEM) Cl⁻ and Ca²⁺ responses elicited by H1R agonist or histamine in the absence or presence of CFTR_inh-172. The symbols connected by lines in (b) and (c) denote data from the same cells. **: p < 0.001