Fig. 5
Effect of blockade/KO of Gβγ, PKA, CFTR or NKCC1 on histamine-induced resetting of circadian clock. a-j Plots against ZT of the firing rate of SCN neurons recorded in different experimental conditions. Each plot shows the representative result of 6 repeated experiments. The projected light and dark phases of the animal room are indicated with open and filled horizontal bars, respectively. The dashed vertical line in each plot indicates the average time of peak neural activity for control slices. The filled square denotes the time of slice preparation, while the arrow indicates the time of drug application. k Graph summarizing the effects of various experimental treatments on the time of peak of circadian firing activity rhythm. Student-Newman-Keuls comparison tests were performed after Kruskal-Wallis one-way ANOVA on Ranks (p < 0.001). The results of pair-wise comparisons of the value of each experimental group with those of control and histamine groups are denoted with asterisk and spade, respectively. *, ♠: p < 0.05