Fig. 2

Down regulation of environmental Wg induces the decrease of PD-Angle. a-b At embryonic stage 14, wg RNA is detected by FISH. Normalized to the segment width, both wg RNA distribution and the distance between the center of wg signal and posterior segmental boundary are comparable to wild type control. White arrow heads in (a) indicate the segment boundaries. The insert cartoons in (b) represent the expressing region of Wg with red oval, the segment region with yellow rectangle, and the parameters quantified in corresponding panels. (n = 15–30). c - d Wg expression level is significantly decreased in wg ^l-12 embryos, while its center-to-boundary distance is not changed after normalized to the segment boundary. (n = 28–32). e-f Knockdown of wg, wntless or vps26 in epithelial cells by wg-Gal4 results in the decreased PD-Angle. (n = 22–49). Scale bars, 10μm in (a) and (c) and 25μm in (c)