Fig. 2

Oxidative stress induced by 6-OHDA treatment reduces phosphorylated PAK1. a SH-SY5Y cells were treated with 50 μM of 6-OHDA and harvested at 0, 3, 6, and 9 h after treatment. The lysates were used to measure phosphorylated PAK1 and total PAK1 levels by Western blot analysis. b Bar graphic representation of Western blot analysis [0 h: 100.0 ± 11.61 %, n = 3; 3 h: 95.4 ± 5.35 %, n = 3; 6 h: 78.2 ± 10.76, n = 3; 9 h: 36.0 ± 14.19 %, n = 3, One-way ANOVA, F _3,11 = 7.070, P = 0.0122, Newman-Keuls multiple comparison test: ^* P < 0.05]. c A 4 μl dose of 6-OHDA (5 μg/μl) was infused into the right striatum at rate of 0.5 μl/min. After 7 days, the midbrain regions including the substantia nigra of the ipsilateral side and contralateral side were isolated and 30 μg of each protein was used for Western blot analysis. d Bar graphic representation of Western blotting analysis (Contra: 100.0 %, n = 3; Ipsi: 68.2 ± 1.56 %, n = 3, Paired t-tests, ** P < 0.001)