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Fig. 3 | Molecular Brain

Fig. 3

From: rAAV-compatible MiniPromoters for restricted expression in the brain and eye

Fig. 3

Eight MiniPromoters in rAAVs show unique expression related to their source gene when driving icre in mice carrying a “lox-stop-lox-lacZ” allele, an historical-indirect reporter. Each tEMS number indicates an individual animal. Ple94-icre (GPR88 RRs) expressed strongly in the striatum and in upper cortical layers. Ple198-icre (SLC6A4 RRs) expressed in the thalamus and shows a clear demarcation between LGd and LGv. Ple251-icre (C8ORF46 RRs) demonstrated strong expression in the hippocampus and cortex; layers IV, V, and Vb are predominant. The zona incerta is also labeled. Ple251-icre-WPRE (C8ORF48 RRs) extended the expression to most brain regions, particularly throughout the cortex and in the cerebellar granular layer. Ple266-icre (S100B RRs) expressed sporadically in the brain with puffy processes in the cortex and corpus callosum. As expected for partial viral transduction, a subset of GFAP+ astrocytes are co-labelled with β-gal. Ple267-icre (UGT8 RRs) has strong staining in the olfactory bulb and in the cerebellar granule layer, with some Purkinje cells labeled. In the OB, globeruli-like structures are stained. Ple303-icre (NOV RRs) stains all cortical layers in midline and becomes specific to cortical layer V in more lateral sections. Ple304-icre and Ple305-icre (both OLIG1 RRs) demonstrated an indistinguishable expression pattern with scattered cells in several brain regions, including the cortex and brainstem, resembling puffy processes of oligodendrocytes. Bs, brainstem; Cb, cerebellum; Ctx, cortex; Hipp, hippocampus; icre, improved cre recombinase; LGd, dorsal lateral geniculate; LGv, ventral lateral geniculate; Mb, midbrain; OB, olfactory bulb; RRs, regulatory regions; Str, striatum; Th, thalamus. Blue, β-gal positive; brown, DAB immunohistochemistry; red, neutral red. [Scale bars = 100 μm]

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