Fig. 1
From: Rescuing macrophage normal function in spinal cord injury with embryonic stem cell conditioned media
Mye-Mϕ formation in vivo and in vitro. a Using Oil Red O (ORO) staining, lipid accumulation at the injury site can be observed starting as diffuse staining at 10 days and by 2 weeks after SCI. There is a central core of high intensity staining at 2 weeks post SCI. b Electron micrographs of macrophages in the injured spinal cord at indicated time points. c In vitro induction of mye-Mϕ. BMDMs (F4/80+) incubated with myelin debris and intracellular lipids were labeled by ORO. 3D reconstruction by confocal microscopy shows the myelin-derived lipids distributed throughout the cell subsequent to myelin treatment (right)