Fig. 5
From: Rescuing macrophage normal function in spinal cord injury with embryonic stem cell conditioned media
ESC-M Enhances Lipid Efflux from Mye-Mϕ Reducing the Formation of Foamy Cells. a BMDMs exocytosing lipids can be visualized with a ring of lipid droplets (ring cells) on the cell periphery. b Mye-Mϕ were treated with CON-M and ESC-M, respectively for indicated time points. The number of foam cells exocytosing lipids was quantified as the percentage of total cells with ring cells (n = 5). c The product of reverse cholesterol transport is free extracellular cholesterol which was quantified with a flourometric assay (n = 4). d Naïve-Mϕ and mye-Mϕ were incubated with CON-M and ESC-M for 24 h. The expression of ABCA1 and ABCG1 were determined by Western Blot. (*p < 0.05) Data are represented as means ± SD