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Fig. 4 | Molecular Brain

Fig. 4

From: Positive geotactic behaviors induced by geomagnetic field in Drosophila

Fig. 4

cry-, pdf- and pyx-GAL4 expressing neurons are necessary for the GMF-induced geotactic positioning. a Schematic representation of JO in the second antennal segment. b, c Expression of GFP in cry-GAL4/UAS-mCD8::GFP and pdf-GAL4/UAS-mCD8::GFP, respectively. Scale bar: 10 μm. d The geotactic positioning of JO-injured flies. The second antennal segments of flies were pinched with fine forceps under CO2 anesthesia 24 h before the tube-positioning assay. Anesthetized flies without JO injury were controls. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005 by ANOVA (n = 10 trials). e, f, g The geotactic positioning of the flies with targeted inhibition of the neurons expressing CRY, PDF, and Pyx, respectively, by expressing TNT under the positive geotactic GMF condition. Control flies expressed impTNT. Error bars: SEM. n.s.: not significant. **, P < 0.01; ***, P < 0.005; ****, P < 0.001 by Student’s t-test. For all the data, n = 10 trials

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