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Fig. 1 | Molecular Brain

Fig. 1

From: Growth arrest specific gene 7 is associated with schizophrenia and regulates neuronal migration and morphogenesis

Fig. 1

Gas7 influenced development stages of cortical neurons through F-BAR domain in vitro. a Immunoblotting revealed the Gas7 levels in the cerebral cortex (left) and hippocampus (right) during development. b Schematic representation of domain organization of Gas7. c Morphological characterization of cortical neurons in early three stages (left). Percentage of cortical neurons transfected with Gas7, pCAG-IRES vector (Mock) and truncates of Gas7 (CC, FCH, WW) in different stages (up row). Percentage of cortical neurons transfected with pSUPER (Mock) and shRNAs target-Gas7 (ShB4) in different stages (below row). d Representative images of cortical neurons at stage-3, which transfected at DIV0 for 3d with different truncates of Gas7, Gas7 and Mock by electroporated transfection. e Immunoblotting in HEK293T cells showed that Gas7 specific shRNAs, ShB4 is efficient to knockdown Gas7 rather than shRNA-resistant Gas7 (synonymous mutation). f Representative images of cortical neurons at stage-3 which transfected at DIV0 for 3d with mock, ShB4 together with GFP. g Quantification of number of the primary neurites (left) and the length of the longest neurite (right) as shown in Fig. 1d. Data represent mean ± SEM. N = 3 (number as indicated) for each group. h Quantification of number of the primary neurites (left) and the length of the longest neurite (right) as shown in Fig. 1f. Data represent mean ± SEM. N = 3 (number as indicated) for each group. Scale bar, 5 μm

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