Fig. 2

Overexpression of Gas7 and △WW can contribute to aberrant neuron morphogenesis by inducing filopodia. a Representative images of cortical neurons transfected at DIV 4 for 3 d with different truncates of Gas7 (△CC, △FCH, △WW), Gas7, pCAG-IRES (Mock) by calcium phosphate transfection. Scale bar, 20 μm. b Quantification of numbers of basal and apical dendrite branch as shown in Fig. 2a. Data represent mean ± SEM. N = 3 (eight neurons) for each group. c Gas7 regulated F-actin and contributed to F-actin assembly. Plasmids were co-transfected with mCherry-UtrCH, a versatile fluorescent probe for actin filaments. Scale bar, 5 μm. d Quantification of normalized intensity as shown in Fig. 2c. Data represent mean ± SEM. N = 3 (9 neuron) for each group, two fragments per neuron and GFP fluorescence intensity as control. e Representative images of cortical neurons transfected with Mock and Gas7 at DIV 0 for 3d, which were acute treated with Cytochalasin D (30 min). f Quantification of length of the longest neurite as shown in Fig. 2e. Data represent mean ± SEM. N = 3 (>100 neuron) for each group. Scale bar, 50 μm