Fig. 3

Overexpression of Gas7 inhibited radial migration of cortical pyramidal neurons by increasing leading process branching. a, b Representative images showing the E14.5 mouse cortices electroporated with overexpress Gas7, Gas7 ^△WW and pCAG-IRES vector (Mock) and examined at P0. Representative coronal brain sections at P0 were stained with antibodies to GFP (green) and counter stained with Hoechst (blue). Scale bar, 100 μm. c, d Numbers of GFP-positive neurons is quantified in SVZ/VZ, IZ and CP zones of brain. Data represent mean ± SEM. N = 4 for each group. e Representative images showing the E14.5 mouse cortices electroporated with indicated Gas7 or mock plasmid and examined at E17.5. Representative coronal brain sections were stained with antibodies to GFP (green), Tbr1 (a 5-layer marker, red) and Hoechst (blue). Enlarged view of electroporated neurons show excessive LP branching in the IZ and SVZ/VZ zones. Scale bars, 100 μm (up row) and 5 μm (below row)