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Fig. 4 | Molecular Brain

Fig. 4

From: Growth arrest specific gene 7 is associated with schizophrenia and regulates neuronal migration and morphogenesis

Fig. 4

Reduction of Gas7 expression affects neuronal migration by extending leading process length. a Representative images showing the E14.5 mouse cortices electroporated with GFP plasmids together with pSUPER vector (Mock), shRNAs targeting Gas7 (ShB4) and shRNA-resistant Gas7 (Rescue) and examined at P0. Majority of transfected neuron by ShB4 were in the IZ or SVZ zones. Migration defect can be partly rescued. Scale bar, 100 μm. b Numbers of GFP-positive neurons is quantified in three brain zones. Data represent mean ± SEM. N = 4 for each group. c Enlarged view of electroporated neurons after depletion of Gas7 show extending LP length, while it can be recused by shRNA-resistant Gas7. Scale bar, 5 μm. d In utero electroporation at E14.5 with ShB4 and Mock as indicated. Representative coronal brain sections were stained with antibodies to GFP (green), Cux1 (a 2–3 layer marker, red). Scale bar, 100 μm

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