Fig. 1
From: Calcium activated adenylyl cyclase AC8 but not AC1 is required for prolonged behavioral anxiety
Paradigm of five sessions of repeated EPM test and behavioral comparison vs one session of EPM. A) C57/Bl mice were tested in the EPM apparatus for 5 min. The interval between each session is 30 min. Mice were retested after 24 h. A group of mice (n = 10) has been evaluated after 21 days. Vehicle or drugs were injected 30 min before the first session. B 1 ) Mice were tested in the elevated plus-maze apparatus for 5 min. The interval between each session was 30 min. Black circular symbols are means ± SEM (error bars) of 22 mice per session. A group of 10 mice were retested after 21 days. Single (*) or double asterisk (**) indicates a significant difference respectively from the (first/first and second) session. B 2 ) Black bars indicate the number of total entries (closed and open) in the arms (means ± SEM represented by error bars) of 22 mice per session. Red bars represent the number (means ± SEM symbolized by error bars) of the entries in the open arms of the same groups in the same sessions. Mice were retested after 24 h. A group of 10 mice were used in the session of 3 weeks. (*/***/****) indicate a significant difference respectively from the (first/first, second and 3 weeks/first, second, third and 3 weeks) session. The hashes (#/##) represent a significant difference in the open arms entries from the (first/first and second) session in the same groups. C 1 ) Mice undergoing five repeated sessions of EPM (black circles; means ± SEM, as error bar) and one time in the EPM (white triangles; means ± SEM, as error bar), both retested after 24 h, are compared. Significant difference, inside the single EPM group, is represented with the (*). The (#) shows a significant difference between single and repeated EPM group for the same session. C 2 ) Black and blue bars represent the number of the total entries (means ± SEM, as error bar) in the first session and after 24 h of mice tested five repeated times or one time in the EPM. Red and striped bars represent the number of the entries in the open arms (means ± SEM, as error bar) of the same groups in the first and 24 h later session. The (*) indicates a significant difference of the same parameter inside the same group (same bar color) before after 24 h. The (#) indicates a significant difference for the same parameter between the two groups after 24 h