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Fig. 4 | Molecular Brain

Fig. 4

From: Glycine confers neuroprotection through microRNA-301a/PTEN signaling

Fig. 4

miR-301a protects against OGD-induced cortical neuronal death. a, The expression of miR-301a in cultured cortical neurons at different time following OGD insult are measured by qRT-PCR. miR-301a is decreased at 2, 6, 12 & 24 h after OGD insult (n = 3, *P < 0.05 vs. Sham, ANOVA test). The data are normalized to that in Sham 1. b, The expression of miR-146a in cultured cortical neurons at different time following OGD insult are measured by qRT-PCR. miR-146a is not altered at 2, 6, 12 & 24 h after OGD insult (n = 3, ANOVA test). The data are normalized to that in Sham 1. c, Treatment of miR-301a agomir reduces OGD-induced cortical neuronal death. Left, LDH assay; Right, MTT assay (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, ANOVA test). d, Treatment of miR-301a antagomir increases OGD-induced neuronal death. Left, LDH assay; Right, MTT assay (n = 6, *P < 0.05 vs. Sham, # P < 0.05 vs. Inj, ANOVA test). Inj, injury; Gly, glycine; Ant, antagomir; Ant-con, antagomir control; Ago, agomir; Ago-con, agomir control

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