Fig. 6

miR-301a directly binds the predicted 3′UTR target sites of PTEN. a, The predicted miR-301a target sequence in the 3′UTR target sites of the PTEN gene. A mutation was generated in the seed region of PTEN 3′UTR as indicated by the underline. b, Luciferase reporter assays in PC12 cells transfected with pMIR-REPORT Luciferase vector carrying PTEN 3′UTR (PTEN wild-type) versus pMIR-mut-PTEN 3′UTR (PTEN mutant) in the absence or presence of miR-301a agomir. The luciferase activity was reduced in the PC12 cells co-transfected with a luciferase reporter containing the PTEN 3′UTR and miR-301a agomir (n = 6, *P < 0.05 vs. miR-301a agomir control, ANOVA test). C, Luciferase reporter assays in PC12 cells transfected with pMIR-REPORT Luciferase vector carrying PTEN 3′UTR (PTEN wild-type) versus pMIR-mut-PTEN 3′UTR (PTEN mutant) in the absence or presence of the miR-301a antagomir. The luciferase activity was increased in the PC12 cells co-transfected with a luciferase reporter containing the pMIR-PTEN 3′UTR and miR-301a antagomir (n = 6, *P < 0.05 vs. miR-301a antagomir control, ANOVA test). Control: untransfected PC12 cells; Blank: PC12 cells transfected with pMIR-REPORT Luciferase vector carrying PTEN wild-type or PTEN mutant without miR-301a agomir or antagomir