Fig. 1
From: Zbtb20 modulates the sequential generation of neuronal layers in developing cortex
Expression of TF Zbtb20 in the developing pallium. a Immunostaining for Zbtb20 at E13.5 demarcates a strong signal just ventral to the cortico-striatal sulcus (white line) in VP and a weaker gradient in LP (arrow) gradually disappearing toward DP. b1-b6 Double-labelling for Pax6 (b1/b4) and Zbtb20 (b2/b5), and an overlay (b3/b6) in VZ of LP and VP at E13.5. c1-c6 Double-labelling for CoupTF1 (c1/c4) and Zbtb20 (c2/c5), and an overlay (c3/c6) in VZ of LP and VP at E13.5. The cortico-striatal sulcus is depicted by a white line. d Zbtb20 IHC on a cross brain section at stage E15.5 demonstrates strong immunosignal in germinative zones of both pallium and subpallim. e1-e4 Triple IHC with antibodies for phospho-vimentin (pVim, E1), Pax6 (e2) and Zbtb20 (e3), and an overlay (e4) in E16.5 DP. Arrows depict triple-positive cells at the apical surface, arrowheads depict a more basally located dividing cell. f IHC for Zbtb20 on coronal WT brain sections at P4. The arrow points to the Zbtb20 expression in the uppermost neocortical layers. The frames depict the position of the micrographs shown in G1-G3. g1-g3 Double immunostaining for Zbtb20 (g1, counterstained by DAPI) and Satb2 (g2), and an overlay (g3) on P4 brain cross sections. Arrows depict double-positive cells in the uppermost layers. h1-h3 Double immunostaining for Zbtb20 (H1, counterstained by DAPI) and Nestin (h2), and an overlay (h3) on P0 brain cross sections depicts almost complete co-staining in VZ/SVZ. Cgl, cingulate cortex; DP, dorsal pallium; LP, lateral pallium; Ncx, neocortex; VP, ventral pallium. Scale bars: a, 100 μm; b3/c3, 20 μm; d, 200 μm; e4, 10 μm; f, 200 μm; g3, 50 μm; b6/c6/h3, 10 μm