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Fig. 5 | Molecular Brain

Fig. 5

From: Zbtb20 modulates the sequential generation of neuronal layers in developing cortex

Fig. 5

Altered cell cycle parameters in Zbtb20 lacZ/lacZ mice. a1-c3 Impaired cell cycle kinetics in DP of Zbtb20 mutant during late neurogenesis at stage E16.5. a1-a3 Decreased proliferation as measured by short - term (40 min) BrdU pulse labelling. Cells were evaluated in frames sized 300 μm (h) × 200 μm (w) in DP. b1-b3 Co-immunostaining for BrdU and Ki67 after 24 h BrdU pulse labelling (E15.5- > E16.5) revealed an increased percentage of cells exiting the cell cycle in the DP of the mutant as compared with the WT cortex. Cells were evaluated in frames sized 300 μm (h) × 100 μm (w) in DP. c1-c3 Labelling with Tbr2 antibody indicates a depletion of intermediate progenitors in Zbtb20 lacZ/lacZ DP. Cells were evaluated in frames sized 300 μm (h) × 200 μm (w) in DP. Statistical analysis (a3,b3,c3) proves that the differences are significant (*, P < 0.05, n = 3 per genotype). d1-d3 Unaltered cell cycle exit kinetics for early (BrdU-tagged at E12.5) progenitors at E13.5. Cells were evaluated in frames sized 150 μm (h) × 75 μm (w) in DP. No change in the percentage of cells exiting the cell cycle in the mutant DP as calculated by BrdU/Ki67 co-staining after a 24 h (E12.5- > E13.5) BrdU pulse (P > 0.05, n = 3 per genotype). Scale bars: 20 μm

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