Fig. 6
From: Zbtb20 modulates the sequential generation of neuronal layers in developing cortex
Regulation of Coup-TF1 expression by Zbtb20. a1-c2 Striking enhancement of the expression of TF CoupTF1 in Zbtb20 lacZ/lacZ mutants. (a1-a4) Ectopic expansion of the CoupTF1 signal in the SS area at P4 as detected by ISH. Note the shift of a strong CoupTF1 signal in a wide band in the ULs of the mutant (arrows in a2). (b1-c2) Ectopic expansion of CoupTF1 expression in mutant DP/MP at stage E12.5, detected either by ISH (b1-b2) or IHC (c1-c2). d1-d2 Zbtb20 binds to the CoupTF1 promoter. d1 The scheme depicts relative positions of fragments on the promoter and the first intron of CoupTF1 that are used in ChIP experiment. d2 ChIP analysis for the CoupTF1 promoter and first intron occupancy by Zbtb20 in cortical neural stem cells from wild type (WT) and Zbtb20 lacZ/lacZ cortices (as negative control). Scale bars: a2, 1 mm; a4/c2, 100 μm