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Fig. 5 | Molecular Brain

Fig. 5

From: Loss of AP-2delta reduces retinal ganglion cell numbers and axonal projections to the superior colliculus

Fig. 5

Loss of Brn3c expression in AP-2δ−/− retina. Paraffin-embedded tissue sections from E16.5 (a) and P1 (b, c) AP-2δ+/+ and AP-2δ−/− retina were processed as previously described [81] and immunostained with two anti-Brn3c antibody: mouse anti-Brn3c (QQ8) (a, b) and rabbit anti-Brn3c antibody (Atlas) (a, c). The signal was detected using the Dako-Cytomation EnVision + anti-rabbit or anti-mouse secondary systems. Tissues were counterstained with the nuclear stain hematoxylin. Photographs were taken using a 20× lens. Images of the entire E16.5 retina sections were assembled from multiple overlapping photographs using the Adobe Photoshop merge function. The bottom panels in a show a higher magnification of the E16.5 ganglion cell layer

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Molecular Brain

ISSN: 1756-6606