Fig. 5

The distribution, organization and number of bipolar cells in the APLP2-KO adult retina differ to WT and APP-KO. Longitudinal cryostat sections of retina were stained for (a-c) Chx10, (d-f) Gγ₁₃ and (g-i) PKCα positive bipolar cells. The dendritic branches appeared diffuse and in some cases cells lacked processes on their outer surface (e and h, arrowheads). l and m mGluR6 (red) has an abnormal staining pattern of bipolar cell processes verified by double staining with PKCα (green). n and o Recoverin stains for Type 2 OFF cone bipolar cells in the INL region (arrowheads). Longitudinal cryostat sections of retinas were immunostained for (p and q) calbindin and (r and s) NF200 and counterstained with DAPI (blue). Immunostained sections from WT (a, d, g, l, n, p and r), APLP2-KO (b, e, h, m, o, q and s) and APP-KO (c, f and i) retinas were compared. Scale bars: 80 μm (a-c); 40 μm (d-f); 15 μm (g, h, i, l and m); 50 μm (n and o); 70 μm (p and q); 10 μm (r and s). Quantification of (j) bipolar cells and (k) type 2 OFF cone bipolar and horizontal cells in WT, APLP2-KO and APP-KO retina performed by counting number of positive nuclei per 400 μm field. Mean ± s.e.m., N = 3 mice per genotype. **, p < 0.01; ***, p < 0.005. (t-test). (t) Western blot analysis of WT and APLP2-KO protein extracts of the PN5 and adult retinas were probed for Chx10 (39 kDa), RIBEYE (48 kDa), mGluR6 (95 kDa) and the loading control, actin (43 kDa)