Fig. 4

The mechanisms underlying A1AR effects in SBI. a Western blot analysis showing phosphorylation level of ERK1/2 and JNK in the sham, ICH, ICH + R-PIA, and ICH + 8-PT groups at 48 h after ICH onsets. b Western blot analysis showing expression of p-P38, p-Hsp27, and p-MAPKAP2 in the sham, ICH, ICH + R-PIA, and ICH + 8-PT groups. c Quantification of the results in panel b. The mean values of the protein levels in the sham group were normalized to 1.0. *p < 0.05 for the ICH group versus the sham group, ^#p < 0.05 for the ICH + R-PIA group versus the ICH group, & p < 0.05 for the ICH + 8-PT group versus the ICH group. d, e Western blot analysis showing expression of p-P38, p-Hsp27, and p-MAPKAP2 in vitro. *p < 0.05 for the OxyHb group versus the control group, ^#p < 0.05 for the OxyHb + R-PIA group versus the OxyHb group, & p < 0.05 for the OxyHb + 8-PT group versus the OxyHb group. f Following treatment with the P38 (SB203580) and Hsp27 (KNK437) antagonists, we detected changes in levels of p-P38, p-Hsp27, and p-MAPKAP2 in vivo at 48 h after ICH onsets. g Quantification of the results in panel f. The mean values of the protein levels in the ICH group were normalized to 1.0. *p < 0.05 for the ICH + R-PIA group versus the ICH group, ^#p < 0.05 for the ICH + R-PIA group versus the ICH + R-PIA + KNK group, & p < 0.05 for the ICH + R-PIA group versus the ICH + R-PIA + SB group