Fig. 4
From: Acid Sensing Ion Channels (ASICs) in NS20Y cells – potential role in neuronal differentiation
cpt-cAMP mediated neurite extension is reduced by ASIC1a knock-down. a Western Blot shows that ASIC1a-shRNA treatment for 72 h significantly decreases ASIC1a expression compared with Control-shRNA treated NS-20Y cells either with or without 1 mM cpt-cAMP treatment. b Example photomicrographs of cpt-cAMP treated cells in the presence of control-shRNA or ASIC1a-shRNA as indicated. Blue arrows indicated NS20Y cells with enhanced neurite extension. cpt-cAMP + ASIC1a-shRNA treated cells have less and decreased neurite length indicated by the blue arrow. Increases in neurite length seen with application of 1 mM cpt-cAMP in control-shRNA transfected cells is significantly reduced when NS20Y is transfected with ASIC1a-shRNA (p < 0.01) (n = 25–44)