Fig. 3

DISC1 is involved in the neuronal activation-induced mitochondrial anchoring. a Retained axonal mitochondrial movement upon neuronal activation by DISC1 overexpression. Primary cortical neurons were transfected as indicated followed by time-lapse imaging before and after treatment of 100 mM KCl for 5 min at DIV 10. 10 mM EGTA was treated for 20 min before KCl stimulation. Scale bar; 10 μm. i) Representative kymographs and ii) quantitative analyses were shown (n = 33 axons, 383 mitochondria for control, n = 32 axons, 395 mitochondria for control with EGTA, n = 34 axons, 446 mitochondria for FLAG-mDISC1, and n = 34 axons, 380 mitochondria for mSNPH shRNA). b Somatic calcium dynamics upon DISC1 or SNPH knockdown in primary cortical neurons at DIV 10. i) Calcium response graph under 100 mM KCl stimulation. ii) Statistical analyses based on peak amplitudes and average area of the graph (n = 43 for control shRNA, n = 45 for mDISC1 shRNA, and n = 42 for mSNPH shRNA). Error bars are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant, by one-way ANOVA with Bonferroni’s multiple comparison test