Fig. 1
From: Astrocyte-secreted thrombospondin-1 modulates synapse and spine defects in the fragile X mouse model
Spine length is altered in Fmr1 KO hippocampal neurons at 17 DIV. a Representative images of DiI labeled WT (left panel) and Fmr1 KO (right panel) neurons highlighting dendrites and spines. Inset is an example of a 50 μm segment used for morphological analysis and quantification. Note the high prevalence of filopodium-like spines in the Fmr1 KO dendritic segment (right panel) relative to the WT segment (left panel). b Cumulative frequency distribution of spine lengths comparing Fmr1 KO and WT neurons. c Fmr1 KO neurons display increased dendritic spine length compared to their WT counterparts. d Assessment of spine morphology in Fmr1 KO neurons shows a significant increase in the number of filopodia-like spines and e a reduction in the number of stubby spines. Data was analyzed by Student’s t-test. Data are presented as the mean ± SEM, *p < 0.05, **p < 0.01; n = 75 neurons per group, N = 3 independent experiments. Scale bars: 10 μm