Fig. 6
From: Orchestrated activation of mGluR5 and CB1 promotes neuroprotection
CDPPB, URB597 and JZL184 treatment are unable to activate ERK1/2 and AKT and promote neuroprotection of mGluR5−/− neurons. a Graph shows cell death levels of corticostriatal neurons from either mGluR5+/+ or mGluR5−/− embryos that were either untreated (−) or treated (+) with 50 μM glutamate, 100 nM CDPPB, 1 nM URB597 and 10 nM JZL184 for 4 h. Data represent the means ± SEM of four independent experiments. * indicates significant difference as compared to matched treated mGluR5+/+ neurons (p <0.05). Shown are representative immunoblots for phospho- (upper panel) and total-ERK1/2 expression (lower panel) and graphs depicting the densitometric analysis of phospho-ERK1/2 normalized to total- ERK1/2 expression in primary cultured corticostriatal neurons from either mGluR5+/+ (b) or mGluR5−/− (c) embryos that were either untreated (−) or treated (+) with 50 μM glutamate, 100 nM CDPPB, 1 nM URB597 and 10 nM JZL184 for 7.5 min. Also shown are representative immunoblots for phospho- (upper panel) and total-AKT expression (lower panel) and graphs depicting the densitometric analysis of phospho-AKT normalized to total-AKT expression in primary cultured corticostriatal neurons from either mGluR5+/+ (d) or mGluR5−/− (e) embryos that were either untreated (−) or treated (+) with 50 μM glutamate, 100 nM CDPPB, 1 nM URB597 and 10 nM JZL184 for 7.5 min. 100 μg of cell lysate was used for each sample. Data represent the means ± SEM of four independent experiments. n.s. indicates not significant and * indicates significant difference as compared to untreated neurons (p <0.05)