Fig. 9
From: Orchestrated activation of mGluR5 and CB1 promotes neuroprotection
CDPPB protects postsynaptic terminals and JZL184 mainly protects the presynaptic site. Shown are laser-scanning confocal micrographs from neurons immunolabeled for PSD95 (green), anti-syntaxin 1A (red) and DAPI (blue), which were either untreated (NC) (a) or treated with 50 μM glutamate (glut) in the absence (b) or in the presence of 100 nM CDPPB (c) and 10 nM JZL184 (d) for 4 h. Scale bar = 20 μM. Graphs show average intensity of pixel grey levels of PSD95 (e) and syntaxin 1A (f) staining, obtained from at least five images taken from four independent experiments. Neurons were either untreated (−) or treated (+) with 50 μM glutamate, 100 nM CDPPB, 10 nM JZL184, 1 μM MPEP or 10 nM AM251, for 4 h. Data represent the means ± SEM. * indicates significant difference as compared to glutamate treated neurons and # indicates significant difference as compared to untreated neurons (p <0.05)