Fig. 1

Schematic neural induction diagrams and characterization of the NSPCs generated from human PBMC-derived iPSCs. a Schematics of the NSPC induction protocols used in this study. (Scale = 200 μm for the images of neurospheres.) (b, c, d) Representative data taken by 1210B2-NSPCs for characterization analysis of the NSPCs. b Cell surface markers of the induced NSPCs. c The quantitative RT-PCR analysis results are depicted by ΔCt values. Quantitative RT − PCR analysis confirmed the decrease in the iPSC markers, and an increase in NSPC markers following the differentiation of iPSCs into NSPCs. (n = 2 for each iPSC-NSPC lines) d Representative immunocytochemistry data collected to confirm the differentiation potential of NSPCs into neuronal and glial lineages. (Scale = 100 μm.) e The correlation coefficients of the expression profiles among the NSPCs. The microarray profiles were similar between all the NSPCs regardless of the induction protocols or iPSCs. (n = 2 for each iPSC-NSPC lines) See also Additional file 1: Figure S1 and Additional file 2: Table S1