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Fig. 8 | Molecular Brain

Fig. 8

From: In vivo whole-cell recording with high success rate in anaesthetized and awake mammalian brains

Fig. 8

Intracellular pharmacology by conventional whole-cell recording. (a) Example cells showing that including QX314 in internal solution was able to block neuronal firing. Cells were recorded in voltage-clamp mode from CA1 pyramidal layers of anaesthetized rats. Without drug treatment (two cells shown in top panel), neuronal firing was elicited by depolarizing the cell to –35 mV (from –70 mV); in the presence of QX314 (two cells shown in bottom panel), neuronal firing could not be elicited by the same depolarization. (b, c) Blocking effect of intracellular application of MK801 on complex spikes that occurred spontaneously in neurons of CA1 pyramidal layers (data obtained from anaesthetized rats). Example traces (b; with low and high temporal resolutions shown in top and bottom panels, respectively) for simple (standard) spikes and complex spikes, which were recorded in the absence of MK801 (in current-clamp mode at resting potentials). Compared with simple spikes, complex spikes consisted of more (4―6) APs and a remarkably larger and slower depolarization (LSD). Summary of the frequencies of complex spikes recorded in the absence and presence of intracellular application of MK801 is shown in (c) (n = 18 each). *** P < 0.001

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