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Fig. 1 | Molecular Brain

Fig. 1

From: Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines

Fig. 1

Establishment and characterization of LCL-derived iPSCs. a Representative image of an LCL culture. Scale bar = 100 μm. b iPSCs derived from healthy control LCLs (LKA10, LKA29 and LKA36), isogenic dermal fibroblasts (DFs) (eKA3, KA11, and KA23) and LCLs from a PARK2 patient (LPB1, LPB3 and LPB7) were immunopositive for the pluripotent markers OCT4 (green) and TRA-1-60 (red). Scale bar = 200 μm. ce The expression levels of pluripotent markers NANOG and OCT4 in LiPSCs (LKA10, LKA29 LKA36, LPB1, LPB3 and LPB7), DF-iPSCs (eKA3, KA11 and KA23) and LCLs (LCL(KA) and LCL(PB)) were assessed by quantitative reverse-transcription PCR (qPCR). The values from the previously established DF-iPSCs (201B7, a previously established human iPSC clone [5]) were set to 1.0 (n = 3 independent experiments; means ± SEM; n.s., not significant; Student’s t-test). f The expression levels of EBV-related genes (EBNA-1, EBNA-2, BZLF-1, LMP-1 and OriP) were analyzed by a PCR analysis of the genomic DNA obtained from parental LCLs and LCL-derived iPSCs. GAPDH was used a loading control. g Comparison of the global gene expression profiles of DF-iPSCs (eKA3 and KA11), LiPSCs (LKA29, LKA36, LPB1 and LPB7), TiPSCs (TKA4 and TKA9) [6], and the original cells (DF(KA), LCL(KA), LCL(PB) and T-cell(KA)). Principal component analysis of the gene expression data. Black: DF, Brown: LCLs, Yellow: T-cell, Green: DF-iPSCs, Red: LiPSCs, Blue: TiPSCs. h Hierarchical clustering analysis of the global gene expression profiles. The data discussed in this publication have been deposited in the NCBI Gene Expression Omnibus (GEO, http://www.ncbi.nlm.nih.gov/geo/) database and are accessible through GEO Series accession numbers GSE76832 [6] and GSE82159

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