Fig. 1

G_q protein-coupled intracellular Ca²⁺ ([Ca²⁺]_i) rise is impaired by acute PERK inhibition. a [Ca²⁺]_i of primary cortical neurons in response to 250 μM carbachol treatment (DMSO n = 21, PI n = 17; ***p < 0.001, two-tailed student’s t-Test). b [Ca²⁺]_i of primary cortical neurons in response to 50 μM DHPG treatment (DMSO n = 36, PI n = 57; ***p < 0.001, two-tailed student’s t-Test). c [Ca²⁺]_i of primary cortical neurons in response to 1 μM bradykinin treatment (DMSO n = 25, PI n = 37; ***p < 0.001, two-tailed student’s t-Test). In all of the experiments above, cells were pretreated with 500 nM PERK inhibitor (PI) or DMSO for 15 min before recording. In the representative graph on the left, each Ca²⁺ trace represents the average of 6–11 neurons that were imaged from the same coverslip. Basal Ca²⁺ oscillation over 100 sec before treatment and drug-stimulated [Ca²⁺]_i rise over 200 sec were quantified by calculating the area under the curve (AUC). Final analysis is presented as AUC/100 sec and shown in the bar graph on the right