Fig. 3

Acute PERK inhibition increases IP₃ receptor mediated ER Ca²⁺ release. a [Ca²⁺]_i. of primary cortical neurons in response to 250 μM carbachol treatment in Ca²⁺ free bath (DMSO n = 29, PI = 26; * p < 0.05, two-tailed student’s t-Test). b [Ca²⁺]_i. of primary cortical neurons in response to 50 μM DHPG treatment in Ca²⁺- free bath (DMSO n = 33, PI = 39; * p < 0.05, two-tailed student’s t-Test). In both experiments, cells were pretreated with 500 nM PERK inhibitor (PI) or DMSO for 15 min before recording. Drug treatment started 100 sec after Ca²⁺- free bath perfusion. In the representative graph on the left, each Ca²⁺ trace represents the average of 8–12 neurons that were imaged from the same coverslip. Basal Ca²⁺ oscillation over 100 sec before treatment and drug-stimulated [Ca²⁺]_i rise over 20–30 sec were quantified by calculating the area under the curve (AUC), and shown in the middle and right bar graphs respectively