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Fig. 4 | Molecular Brain

Fig. 4

From: PERK regulates Gq protein-coupled intracellular Ca2+ dynamics in primary cortical neurons

Fig. 4

Acute PERK inhibition impairs receptor-operated Ca2+ entry, but not store-operated Ca2+ entry. a [Ca2+]i. of thapsigargin (TG) pretreated primary cortical neurons in response to 50 μM DHPG treatment. Cells were pretreated with 500 nM PERK inhibitor (PI) or DMSO for 15 min before recording, and perfused with 1 μM TG for 300 sec before 50 μM DHPG treatment. In the representative graph on the left, each Ca2+ trace represents the average of 8–9 neurons that were imaged from the same coverslip. Basal Ca2+ oscillation over 100 sec before treatment and DHPG-stimulated [Ca2+]i rise over 500 sec were quantified by calculating the area under the curve (AUC). Final analysis is presented as AUC/100 sec and shown in the bar graph on the right (DMSO n = 37, PI n = 35; *** p < 0.001, two-tailed student’s t-Test). b Store-operated Ca2+ entry in primary cortical neurons. Cells were pretreated with 500 nM PI or DMSO for 15 min before recording, and perfused with 1 μM TG in Ca2+- free bath for 300 sec before reintroduction of 2 mM Ca2+. In the representative graph on the left, each Ca2+ trace represents the average of 9–12 neurons that were imaged from the same coverslip. Store-operated Ca2+ entry over 500 sec was quantified by calculating the area under the curve (AUC). Final analysis is presented as AUC/100 sec and shown in the bar graph on the right (DMSO n = 45, PI n = 36; n.s. not significant, two-tailed student’s t-Test)

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