Skip to main content
Fig. 4 | Molecular Brain

Fig. 4

From: ApCPEB4, a non-prion domain containing homolog of ApCPEB, is involved in the initiation of long-term facilitation

Fig. 4

ApCPEB4, but not ApCPEB is critical for the initiation of LTF. a The expression and induction of ApCPEB4 following 10 μM 5-HT stimulation was blocked by dsApCPEB4. (Upper) Representative images of neurites of cultured sensory neurons immunostained against ApCPEB4. dsApCPEB showed no effect on the ApCPEB4 expression and induction, while dsApCPEB4 significantly blocked ApCPEB4 expression and induction. Scale bar, 40 μm. (Lower) Bar graphs represent the percent fluorescence intensity of ApCPEB4 in the neurites of naïve, dsApCPEB-injected, dsApCPEB4-injected sensory neurons. 5-HT treatment significantly induced the ApCPEB4 expression which was blocked by injection of dsApCPEB4. **, p < 0.01; *, p < 0.05; N.S., not significant, two-tailed unpaired t test. b LTF at 24 h was specifically blocked by knock-down of ApCPEB4 (dsApCPEB4). dsApCPEB or dsLuci showed no effect on the 24 h LTF. (Left) Representative EPSP traces before and 24 h after the 5 pulses of 5-HT treatment at the sensory-to-motor synapses. (Right) Bar graph represents the means ± SEM of the percent change in EPSP amplitude. *, p < 0.05 compared with that of dsLuci group, one-way ANOVA; F = 3.83, Tukey’s post-hoc test. N.S. not significant (c) Overexpressed 3×Flag-ApCPEB4 in cultured sensory neurons was detected by anti-Flag antibody. As a control, EGFP-expressing sensory neurons were used. Scale bar, 20 μm. d The overexpression of ApCPEB4 induced LTF by 1× 5-HT treatment. As a control, EGFP was expressed. Bar graph represents the means ± SEM of the percent change in EPSP amplitude. **, p < 0.01, two-tailed unpaired t test

Back to article page