Fig. 5
From: The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture
Enhanced thalamocortical oscillations in PLCβ4−/− slices. a Schematic of the recording configuration. Extracellular multiunits were recorded in the ventrobasal (VB) region of the thalamus and included the ventral posteromedial and ventral posterolateral nuclei. A 20- to 100-μV and 60- to 80-μs square pulse stimulus was applied to the internal capsule through a bipolar electrode in the horizontal slices from the PLCβ4+/+ and PLCβ4−/− mice with or without cutting the cortical part. b The representative traces exhibit the electrically evoked intrathalamic oscillations that were composed of multiunit activities that were detected in the VB nuclei from the PLCβ4+/+ and PLCβ4−/− slices without the cortical part. The traces were bandpass-filtered at 5 Hz–5 kHz. c The durations of the evoked oscillatory activities were greatly increased in the PLCβ4−/− slices without the cortical part. All of the data from 7 slices from 5 PLCβ4+/+ mice and 10 slices from 6 PLCβ4−/− mice are presented as mean ± SEM. **, p < 0.01