Fig. 2
From: Expression of acid-sensing ion channels and selection of reference genes in mouse and naked mole rat
Ranking of housekeeping genes based on their expression stability between tissue samples (2 biological replicates per tissue), from low stability (left) to high stability (right). a Mouse geNorm experiment showing CANX and RPL13A to be the most stably expressed transcripts; M represents variation between samples based on pairwise expression ratio, a lower value means lower variability. b Mouse data showing the optimal number of reference genes for an experiment using samples from all tissues analyzed; V indicates if addition of more housekeeping genes, starting with the two most stable genes from plot 1, increases or decreases variation (a value below 0.15 is recommended). c Naked mole-rat geNorm experiment (2 biological replicates per tissue), EIF4A2 and β-ACTIN were the most stably expressed transcripts. d Naked mole-rat data showing the optimal number of reference genes for an experiment using samples from all tissues analyzed