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Fig. 1 | Molecular Brain

Fig. 1

From: Tat-HSP22 inhibits oxidative stress-induced hippocampal neuronal cell death by regulation of the mitochondrial pathway

Fig. 1

Construction and purification of Tat-HSP22 protein. a Diagram of expressed Tat-HSP22 and control HSP22 proteins. Tat-HSP22 expression vector was constructed using pET15b vector. Purification of Tat-HSP22 and control HSP22 protein. After proteins were expressed by IPTG, the proteins were purified using Ni-NTA affinity chromatography. Then, purified proteins were analyzed by b 12% SDS-PAGE and c Western blot analysis with an anti-histidine antibody

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Molecular Brain

ISSN: 1756-6606