Fig. 1
Alteration of GC maturation stage markers in the DG after ECS. a , Representative images of in situ hybridization of Calb1 at 6 h after single ECS or sham (CNT) treatment. Scale bars: upper 1 mm, lower 200 μm. b, The relative expression of Calb1 and Tdo2 at 6 h after the indicated number of ECS (Dunnett’s test following one-way ANOVA: F (3,12) = 69.72, *** P < 0.001 for Calb1, F (3,12) = 67.15, *** P < 0.001 for Tdo2). c, The relative expression of Dsp and Il1r1 after 11 ECS sessions (t (6) = 5.733, ** P < 0.01 for Dsp, t (6) = 4.418, ** P < 0.01 for Il1r1). d, Representative images of immunoreactivity for NeuN, calbindin, and calretinin in DG at 24 h after 11 ECS sessions. GCL: granule cell layer. Scale bar: 20 μm. e, Immunoblot detections of reduced calbindin D-28 K expression (t (8) = 6.899, *** P < 0.001) and intact PSD-95 expression in DG (t (8) = 1.082, P = 0.31). The n number is given in graph. Data are presented as means ± SEM