Fig. 2
Altered stimulus-induced gene expression in DG after repeated ECS. a, Left: representative images of immunoreactivity for c-fos and NeuN at 2 h after the last ECS. Scale bar, 100 μm. Right: the sham (CNT) treatment or ECS was applied to naive mice or mice treated with 10 times of chronic ECS (cECS). The expression of Fos at 1 h after the last treatment was examined (Tukey’s test following one-way ANOVA: F (3,12) = 35.77, *** P < 0.001). b, The relative expression of other IEGs (Gadd45b, Nr4a1, Arc, and Egr1) mRNA at 1 h after the last ECS or control treatment (Tukey’s test following one-way ANOVA: F (3,12) = 31.16, *** P < 0.001 for Gadd45b, F (3,12) = 38.36, *** P < 0.001 for Nr4a1, F (3,12) = 33.39, *** P < 0.001 for Arc, F (3,12) = 39.82, ** P < 0.01, *** P < 0.001 for Egr1). c, The number of genes that showed a significant increase or decrease by a single ECS-treatment is represented in bars. The three groups that were categorized according to expression change by repeated ECS were color-coded. The n number is given in graph. Data are presented as means ± SEM