Fig. 7
Augmentation of GABAergic signalling reverses long-lasting phenotypic change. a, No significant difference was seen in frequent facilitation at the MF synapse between mice treated with diazepam (DZP, 5 mg/kg) and vehicle (Veh) for 3 weeks. b, Frequency facilitation at 14 days after 11 times of ECS in mice treated with 5 mg/kg diazepam or vehicle (*** P < 0.001, Bonferroni’s test following two-way ANOVA, interaction [drug treatment × ECS]; F (1,17) = 7.39, P = 0.0146). c, The relative expression of Calb1 and Tdo2 at 1 day or 14 days after 11 times of ECS in mice treated with 10 mg/kg diazepam (Tukey’s test following one-way ANOVA: F (2,6) = 122.1, *** P < 0.001 for Calb1, F (2,6) = 90.9, * P < 0.05, *** P < 0.001 for Tdo2). d, Effects of diazepam on frequency facilitation at 14 days after 11 times of ECS. Diazepam (5 mg/kg) was administered during the period of ECS treatments (t (10) = 4.062, P = 0.0097). e, Effects of diazepam (10 mg/kg) administered after 11 times of ECS on the lasting reduction of frequency facilitation (t (10) = 2.873, P = 0.0166). The number (n) is shown in the graph. Data are presented as means ± SEM