Fig. 1

TREM2 is upregulated in OGDR and MCAO ischemic models. TREM2 levels in primary mouse microglia 0, 3, 6, 12, 24, 48, and 72h after OGDR as determined by quantitative real-time PCR (a) and western blotting (b). c Densitometric analysis of TREM2 (relative to GAPDH) from western blotting data in (b) presented as mean ± SEM; n = 6; *P < 0.05 compared with control. TREM2 levels in mice 6h, 1d, 3d, 7d, 14d, 21d and 28d after MCAO were detected by quantitative real-time PCR (d) and western blotting (e). f Densitometric analysis of TREM2 relative to GAPDH from data in (e) presented as mean ± SEM; n = 6; ^# P < 0.05 compared with sham